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DNA ligase is often a kind of enzyme that facilitates the signing up for of DNA strands with each other by catalyzing the development of a website phosphodiester bond. It plays a job in restoring single-strand breaks in duplex DNA in living organisms, but some kinds (which include DNA ligase IV) may possibly exclusively maintenance double-strand breaks (i.
A ligation reaction is most successful when the sticky finishes are already stably annealed, and disruption with the annealing finishes would therefore cause low ligation effectiveness. The shorter the overhang, the lower the Tm.